Dr. Ashish Srivastava
I am a Virologist and majorly working on
Host-virus interaction
Virus-based CRISPR/Cas9 delivery vector development for genome editing
CRISPR/Cas based diagnostics development
I completed my Ph.D. from CSIR-National Botanical Research Institute, Lucknow, India in Plant Molecular Virology. Later, I moved to the University of Delhi South Campus, New Delhi. I also visited South Dakota State University, USA and University of Edinburgh, UK. Now, I am research faculty at Amity Institute of Virology & Immunology, Amity University, Noida. Here, I am running two funded research projects on CRISPR/Cas9 delivery and diagnostic methods development. I am totally committed to my work and upholding the tradition of scientific inquiry. Get in touch if you’d like to learn more about my research interests, my past and current work, or just want to say hello. I look forward to hearing from you.
My Research
From Theory to Reality
Machine learning and Deep learning approach for automatic diagnosis
We at Amity Institute of Virology & Immunology are developing Deep learning & Machine learning based algorithm for automatic virus diagnosis
Virus induced Programmed cell death
We are continuously working on the virus-host interaction. Now, we are trying to establishing the role of plant alkaloids in Programmed cell death.
https://www.frontiersin.org/articles/10.3389/fpls.2017.01172/full
Next Generation Diagnosis of Respiratory Virus and Virus based CRISPR/Cas9 delivery vectors
CRISPR/Cas mediated diagnostics for Respiratory viruses under an Indian Council of Medical Research Funded project. Geminiviruses can be proved a better plant genome editing vectors for CRISPR/Cas9 delivery and enhanced efficiency of HDR (homology direct repair). We are working on Geminiviruses and their associated satellite molecules to modify them as CRISPR/Cas9 delivery vectors under DST funded project.
Off-target Prediction and Overcoming strategies in CRISPR/Cas9-mediated Genome Engineering
CRISPR/Cas9 off-targets are an intensively researched area in the scientific community. A coherent and concise method increasing target precision would prove indispensable to concrete manipulation and interpretation of genome editing results that can revolutionise therapeutics, including clarity in genome regulatory mechanisms during development.
Publications and Awards
10 Years of Research
I’ve been fortunate to have worked on several different research projects in an array of interesting subjects, and I’m happy to say that my hard work paid off, and I have many scientific publications to show for it. Check out my list of published works below and contact me with any questions or for more information about my research.
Top publications
Googlescholar: https://scholar.google.com/citations?hl=en&user=I01RXoEAAAAJ&view_op=list_works&sortby=pubdate
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1. Srivastava A, Gupta T, Srivastava S, Dhir S, Kumar P, Singhal T, Rani A, Rishi N. (2022) Development of a new Collateral Cleavage-independent CRISPR/Cas12a based easy detection system for plant viruses. Journal of Virological Methods. 300:114432
2. Debashree I, Roy R, Rishi N., *Srivastava A. (2021) Advances made in deciphering and minimizing CRISPR/Cas9 Off-targets: causes, prediction, detection and overcoming strategies. Current Bioinformatics (In press: BMS-CBIO-2021-18).
3. Joshi C., Kaushik M., Dutta MK, Srivastava A., Choudhary N. (2021) Automatic Analysis and Viral Disease Diagnosis Using Deep-Learning in Vigna Mungo Plant". Ecological Informatics. 61: 101197.
4. Srivastava et al., (2020) Next-Generation Molecular Diagnostics Development by CRISPR/Cas tool: rapid detection and surveillance of viral disease outbreaks. Front. Mol. Biosci. doi: 10.3389/fmolb.2020.582499.
5. Raj et al., (2020) Sequence identity and phylogenetic analysis of seven potyvirus isolates revealed association of diverse potyvirus species with mosaic and yellow leaf stripe disease in Indian narcissus (Narcissus tazetta) cv. Paper white. 3 Biotech 10, 428 (2020). https://doi.org/10.1007/s13205-020-02418-8.
6. Kumar et al, (2020) CRISPR-Cas system: A novel option for COVID-19 diagnostics and therapeutics. Front Cell Infect Microbiol. 10: 576875.
7. Srivastava, et al., (2017). Ageratum enation virus infection in Papaver sominiferum disorders plant's architecture, affects alkaloid and metabolite biosynthesis, and accelerates programmed cell death. Frontiers in Plant Science. 8:1172.
8. Kumar et al., (2016). Paenibacillus lentimorbus inoculation enhances tobacco growth and extenuates the virulence of Cucumber mosaic virus. Plos One. e0149980.
9. Srivastava et al., (2015). Characterization of an unidentified begomovirus associated with yellow mosaic disease of three ornamental Jatropha species grown in India. Virus Research. 201 (2015): 41-49
10. S. Srivastava, H. Bisht, O.P. Sidhu, Ashish Srivastava, P. C. Singh, R.M. Pandey, S. K. Raj, R. Roy, C.S. Nautiyal (2012). Changes in the metabolome and histopathology of Amaranthus hypochondriacus L. in response to Ageratum enation virus. Phytochemistry 80: 8-16.
Awards and Research Grants
1.     Best Oral Presentation Award February 2020 at International Conference VIROCON 2020 on the theme "Evolution of Viruses and Viral Diseases" during February 18-20, 2020.
2.     INSA-DST-INSPIRE Faculty July-2017 awarded. Faculty Registration No.: IFA17-LSPA100
3.     $150000 grant from DST on virus-based CRISPR/ Cas delivery vector development
4.     $ 20000 grant from ICMR for diagnostics development for influenza and other viruses.
5.     SERB Overseas fellowship for 2017-18 at Plant Pathology Department, University of Edinburgh, Scotland, UK.
6.     ARO Postdoctoral Fellowship for 2015-16 (MARD, ARO, Israel) under Prof. Amit Gal-On, Agricultural Research Organization, The Volcani Centre, Bet-Dagan, 50250, Israel. Title: Development of broad spectrum begomovirus resistant non-transgenic Arabidopsis using CRISPR-Cas9 technique.
7.     Dr. D. S. Kothari Postdoctoral fellowship-2015-2018 (UGC-New Delhi) under Prof. Indranil Dasgupta, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
Awards and Research Grants
1.     Best Oral Presentation Award February 2020 at International Conference VIROCON 2020 on the theme "Evolution of Viruses and Viral Diseases" during February 18-20, 2020.
2.     INSA-DST-INSPIRE Faculty July-2017 awarded. Faculty Registration No.: IFA17-LSPA100
3.     $150000 grant from DST on virus-based CRISPR/ Cas delivery vector development
4.     $ 20000 grant from ICMR for diagnostics development for influenza and other viruses.
5.     SERB Overseas fellowship for 2017-18 at Plant Pathology Department, University of Edinburgh, Scotland, UK.
6.     ARO Postdoctoral Fellowship for 2015-16 (MARD, ARO, Israel) under Prof. Amit Gal-On, Agricultural Research Organization, The Volcani Centre, Bet-Dagan, 50250, Israel. Title: Development of broad spectrum begomovirus resistant non-transgenic Arabidopsis using CRISPR-Cas9 technique.
7.     Dr. D. S. Kothari Postdoctoral fellowship-2015-2018 (UGC-New Delhi) under Prof. Indranil Dasgupta, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
My Team
Research Scholars
